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il-1a cytokine  (PeproTech)


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    Structured Review

    PeproTech il-1a cytokine
    Il 1a Cytokine, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il-1a+cytokine/il+1%CE%B2/pm40325855-51-0-6
    Average 90 stars, based on 1 article reviews
    il-1a cytokine - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin.
    Article Snippet: Cytokine treatments on astrocytes and BMEC-like cells Toprepare conditionedmedium, astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNF (10 ng/ ml; Peprotech), andC1a (500ng/ml;MyBioSource) for 72 h. After three washes with DPBS, cells were placed in minimal conditioning medium containing phenol-red-free DMEM/F12 (Thermo Fisher Scientific) and Glutamax (Gibco).

    Article Title: Cancer Cells Promote Immune Regulatory Function of Macrophages by Upregulating Scavenger Receptor MARCO Expression.
    Article Snippet: RESEARCH ARTICLE | MAY 22 2023 Cancer Cells Promote Immune Regulatory Function of Macrophages by Upregulating Scavenger Receptor MARCO Expression Chao Gu; ... et. al J Immunol (2023) 211 (1): 57–70. https://doi.org/10.4049/jimmunol.2300029 Related Content Disparate Regulation and Function of the Class A Scavenger Receptors SR-AI/II and MARCO J Immunol (December,2005) Regulation and Functional Involvement of Macrophage Scavenger Receptor MARCO in Clearance of Bacteria In Vivo J Immunol (January,1999) Role of macrophage scavenger receptor MARCO in innate immunity against In uenza (133.29) J Immunol (April,2009) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/211/1/57/1646662/ji2300029.pdf by U niversity of Bristol Library user on 27 January 2024

    Article Title: Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin
    Article Snippet: To prepare conditioned medium, astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNF (10 ng/ml; Peprotech), and C1a (500 ng/ml; MyBioSource) for 72 h. After three washes with DPBS, cells were placed in minimal conditioning medium containing phenol-red-free DMEM/F12 (Thermo Fisher Scientific) and Glutamax (Gibco).

    Article Title: Neurotoxic amyloid β-peptide and tau produce cytokine-like effects on PMCA in glioblastoma cell lines, enhancing its activity and isoforms expression.
    Article Snippet: IL-1a and TNF-a were supplied by PeproTech , Thermo Fisher Scientific (Madrid, Spain) and complement component C1q was from Bio-Rad (Hercules, CA, USA).

    Article Title: Cryopreservation of peripheral blood mononuclear cells using uncontrolled rate freezing.
    Article Snippet: Peripheral blood mononuclear cells are widely used as source material for anticancer immunotherapies.. The conventional cryopreservation method for peripheral blood mononuclear cells is time-consuming and expansive, which involves controlled rate freezing followed by storage in liquid nitrogen.. Instead, the convenient uncontrolled rate freezing cryopreservation method had been reported successfully in peripheral blood hematopoietic stem cells and peripheral blood progenitor cells.

    Article Title: Induction of astrocyte reactivity promotes neurodegeneration in human pluripotent stem cell models.
    Article Snippet: To induce a reactive phenotype, hPSC-derived astrocytes were incubated with TNF-a (30 ng/mL, PeproTech), IL-1a (3 ng/mL, PeproTech), and C1q (400 ng/mL, MyBioSource) for 48 h, as previously described (Liddelow et al., 2017).

    Article Title: Transglutaminase 3 Attenuates Skin Inflammation in Psoriasis by Inhibiting NF-κB Activation through Phosphorylated STAT3-TET3 Signaling.
    Article Snippet: Transglutaminase 3 (TGM3) protects against skin inflammation in psoriasis, but the precise role and mechanism of action of TGM3 in the pathogenesis of psoriasis remain unclear.. In this study, we show that TGM3 expression was increased in the skin lesions of patients with psoriasis and a mouse model of imiquimod-induced psoriatic dermatitis.. TGM3 overexpression decreased the production of proinflammatory factors in cultured primary keratinocytes stimulated with psoriasis-related cytokines.

    Article Title: The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1α by Thrombin
    Article Snippet: 2014.024#procedure Other Biopsy punch Kai Medical Cat#BP-40F GSTrap HP column GE Cat#17-5281-01 EpiLife medium ThermoFisher Cat#MEPI500CA CL-XPosure Film ThermoFisher Cat#34089

    Flow Cytometry:

    Article Title: Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin.
    Article Snippet: Cytokine treatments on astrocytes and BMEC-like cells Toprepare conditionedmedium, astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNF (10 ng/ ml; Peprotech), andC1a (500ng/ml;MyBioSource) for 72 h. After three washes with DPBS, cells were placed in minimal conditioning medium containing phenol-red-free DMEM/F12 (Thermo Fisher Scientific) and Glutamax (Gibco).

    Article Title: Cancer Cells Promote Immune Regulatory Function of Macrophages by Upregulating Scavenger Receptor MARCO Expression.
    Article Snippet: RESEARCH ARTICLE | MAY 22 2023 Cancer Cells Promote Immune Regulatory Function of Macrophages by Upregulating Scavenger Receptor MARCO Expression Chao Gu; ... et. al J Immunol (2023) 211 (1): 57–70. https://doi.org/10.4049/jimmunol.2300029 Related Content Disparate Regulation and Function of the Class A Scavenger Receptors SR-AI/II and MARCO J Immunol (December,2005) Regulation and Functional Involvement of Macrophage Scavenger Receptor MARCO in Clearance of Bacteria In Vivo J Immunol (January,1999) Role of macrophage scavenger receptor MARCO in innate immunity against In uenza (133.29) J Immunol (April,2009) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/211/1/57/1646662/ji2300029.pdf by U niversity of Bristol Library user on 27 January 2024

    Article Title: Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin
    Article Snippet: To prepare conditioned medium, astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNF (10 ng/ml; Peprotech), and C1a (500 ng/ml; MyBioSource) for 72 h. After three washes with DPBS, cells were placed in minimal conditioning medium containing phenol-red-free DMEM/F12 (Thermo Fisher Scientific) and Glutamax (Gibco).

    Article Title: Neurotoxic amyloid β-peptide and tau produce cytokine-like effects on PMCA in glioblastoma cell lines, enhancing its activity and isoforms expression.
    Article Snippet: IL-1a and TNF-a were supplied by PeproTech , Thermo Fisher Scientific (Madrid, Spain) and complement component C1q was from Bio-Rad (Hercules, CA, USA).

    Article Title: Cryopreservation of peripheral blood mononuclear cells using uncontrolled rate freezing.
    Article Snippet: Peripheral blood mononuclear cells are widely used as source material for anticancer immunotherapies.. The conventional cryopreservation method for peripheral blood mononuclear cells is time-consuming and expansive, which involves controlled rate freezing followed by storage in liquid nitrogen.. Instead, the convenient uncontrolled rate freezing cryopreservation method had been reported successfully in peripheral blood hematopoietic stem cells and peripheral blood progenitor cells.

    Article Title: Induction of astrocyte reactivity promotes neurodegeneration in human pluripotent stem cell models.
    Article Snippet: To induce a reactive phenotype, hPSC-derived astrocytes were incubated with TNF-a (30 ng/mL, PeproTech), IL-1a (3 ng/mL, PeproTech), and C1q (400 ng/mL, MyBioSource) for 48 h, as previously described (Liddelow et al., 2017).

    Article Title: Transglutaminase 3 Attenuates Skin Inflammation in Psoriasis by Inhibiting NF-κB Activation through Phosphorylated STAT3-TET3 Signaling.
    Article Snippet: Transglutaminase 3 (TGM3) protects against skin inflammation in psoriasis, but the precise role and mechanism of action of TGM3 in the pathogenesis of psoriasis remain unclear.. In this study, we show that TGM3 expression was increased in the skin lesions of patients with psoriasis and a mouse model of imiquimod-induced psoriatic dermatitis.. TGM3 overexpression decreased the production of proinflammatory factors in cultured primary keratinocytes stimulated with psoriasis-related cytokines.

    Article Title: The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1α by Thrombin
    Article Snippet: 2014.024#procedure Other Biopsy punch Kai Medical Cat#BP-40F GSTrap HP column GE Cat#17-5281-01 EpiLife medium ThermoFisher Cat#MEPI500CA CL-XPosure Film ThermoFisher Cat#34089

    Enzyme-linked Immunosorbent Assay:

    Article Title: Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin.
    Article Snippet: Cytokine treatments on astrocytes and BMEC-like cells Toprepare conditionedmedium, astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNF (10 ng/ ml; Peprotech), andC1a (500ng/ml;MyBioSource) for 72 h. After three washes with DPBS, cells were placed in minimal conditioning medium containing phenol-red-free DMEM/F12 (Thermo Fisher Scientific) and Glutamax (Gibco).

    Article Title: Cancer Cells Promote Immune Regulatory Function of Macrophages by Upregulating Scavenger Receptor MARCO Expression.
    Article Snippet: RESEARCH ARTICLE | MAY 22 2023 Cancer Cells Promote Immune Regulatory Function of Macrophages by Upregulating Scavenger Receptor MARCO Expression Chao Gu; ... et. al J Immunol (2023) 211 (1): 57–70. https://doi.org/10.4049/jimmunol.2300029 Related Content Disparate Regulation and Function of the Class A Scavenger Receptors SR-AI/II and MARCO J Immunol (December,2005) Regulation and Functional Involvement of Macrophage Scavenger Receptor MARCO in Clearance of Bacteria In Vivo J Immunol (January,1999) Role of macrophage scavenger receptor MARCO in innate immunity against In uenza (133.29) J Immunol (April,2009) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/211/1/57/1646662/ji2300029.pdf by U niversity of Bristol Library user on 27 January 2024

    Article Title: Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin
    Article Snippet: To prepare conditioned medium, astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNF (10 ng/ml; Peprotech), and C1a (500 ng/ml; MyBioSource) for 72 h. After three washes with DPBS, cells were placed in minimal conditioning medium containing phenol-red-free DMEM/F12 (Thermo Fisher Scientific) and Glutamax (Gibco).

    Article Title: Neurotoxic amyloid β-peptide and tau produce cytokine-like effects on PMCA in glioblastoma cell lines, enhancing its activity and isoforms expression.
    Article Snippet: IL-1a and TNF-a were supplied by PeproTech , Thermo Fisher Scientific (Madrid, Spain) and complement component C1q was from Bio-Rad (Hercules, CA, USA).

    Article Title: Cryopreservation of peripheral blood mononuclear cells using uncontrolled rate freezing.
    Article Snippet: Peripheral blood mononuclear cells are widely used as source material for anticancer immunotherapies.. The conventional cryopreservation method for peripheral blood mononuclear cells is time-consuming and expansive, which involves controlled rate freezing followed by storage in liquid nitrogen.. Instead, the convenient uncontrolled rate freezing cryopreservation method had been reported successfully in peripheral blood hematopoietic stem cells and peripheral blood progenitor cells.

    Article Title: Induction of astrocyte reactivity promotes neurodegeneration in human pluripotent stem cell models.
    Article Snippet: To induce a reactive phenotype, hPSC-derived astrocytes were incubated with TNF-a (30 ng/mL, PeproTech), IL-1a (3 ng/mL, PeproTech), and C1q (400 ng/mL, MyBioSource) for 48 h, as previously described (Liddelow et al., 2017).

    Article Title: Transglutaminase 3 Attenuates Skin Inflammation in Psoriasis by Inhibiting NF-κB Activation through Phosphorylated STAT3-TET3 Signaling.
    Article Snippet: Transglutaminase 3 (TGM3) protects against skin inflammation in psoriasis, but the precise role and mechanism of action of TGM3 in the pathogenesis of psoriasis remain unclear.. In this study, we show that TGM3 expression was increased in the skin lesions of patients with psoriasis and a mouse model of imiquimod-induced psoriatic dermatitis.. TGM3 overexpression decreased the production of proinflammatory factors in cultured primary keratinocytes stimulated with psoriasis-related cytokines.

    Article Title: The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1α by Thrombin
    Article Snippet: 2014.024#procedure Other Biopsy punch Kai Medical Cat#BP-40F GSTrap HP column GE Cat#17-5281-01 EpiLife medium ThermoFisher Cat#MEPI500CA CL-XPosure Film ThermoFisher Cat#34089



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